Comparison of Mycelial Growth of Different Tricholoma matsutake Strains in Soil Medium at Varying Temperatures

Main Article Content

Maki Narimatsu
Muneyoshi Yamaguchi
Takashi Yamanaka
Seiki Gisusi
Tomonori Azuma
Yutaka Tamai
Tohru Fujita
Masataka Kawai


Aims: To investigate the effect of temperature on the mycelial growth of the highly prized mushroom, Tricholoma matsutake, in soil medium.

Methodology: Seven strains of T. matsutake collected from different areas of Japan were incubated in soil medium at 5, 10, 15, 20, 25, and 30°C for 89 day. After incubation, the linear growth of mycelia from the inoculum was measured on vertical lines under a dissecting microscope and classified by the cultivation day number to obtain the growth rate in each vial. The mycelial densities in the soil medium in all the vials were analyzed using qPCR to determine the mycelial biomass.

Results: The mycelial growth of T. matsutake strains was mainly observed at 5–25°C. The optimum temperatures for linear mycelial growth and for the increase in mycelial density were 19.6°C and 17.6°C, respectively. These values were lower than those reported previously for T. matsutake grown on agar medium or in liquid medium. These optimum temperatures showed no clear relationship with the mean air temperature at the site of strain collection.

Conclusion: It can be suggested that a temperature around 18°C is suitable for cultivation of T. matsutake mycelia in the soil medium.

Tricholoma matsutake, linear mycelial growth, mycelial density, optimum temperature.

Article Details

How to Cite
Narimatsu, M., Yamaguchi, M., Yamanaka, T., Gisusi, S., Azuma, T., Tamai, Y., Fujita, T., & Kawai, M. (2019). Comparison of Mycelial Growth of Different Tricholoma matsutake Strains in Soil Medium at Varying Temperatures. Asian Journal of Biotechnology and Bioresource Technology, 5(4), 1-8.
Short Research Article


Imazeki R, Hongo T. Colored illustrations of mushrooms of Japan. In: Imazeki R, Hongo T, Editors. Colored Illustrations of Mushrooms of Japan. Osaka: Hoikuisha Publishing. 1987;1:77. Japanese

Ogawa M. Microbial ecology of mycorrhizal fungus, Tricholoma matsutake Ito et Imai (Sing.) in pine forest, 1: Fungal colony (Shiro) of Tricholoma matsutake. Bull Gov For Exp Stn. 1975;272:79–121. Japanese

Yamanaka K, Aimi T, Wan J, Cao H, Chen M. Species of host trees associated with Tricholoma matsutake and close allies in Asia. Mushroom Sci Biotechnol. 2011;19(2):79–87. Japanese

Vaario LM, Savonen EM, Peltoniemi M, Miyazawa T, Pulkkinen P, Sarjala T. Fruiting pattern of Tricholoma matsutake in Southern Finland. Scand J For Res. 2015;30(4):259–65.

Yamanaka T. Researches for development of the cultivation of ʻmatsutakeʼ, a prized mushroom produced by the ectomycorrhizal basidiomycete Tricholoma matsutake. Bull For For Prod Res Inst. 2012;11(3):85–95. Japanese

Wang Y, Ian RH. Edible ectomycorrhizal mushrooms: Challenges and achieve-ments. Can J Bot. 2004;82(8):1063–73.

Narimatsu M, Koiwa T, Masaki T, Sakamoto Y, Ohmori H, Tawaraya K. Relationship between climate, expansion rate, and fruiting in fairy rings ('shiro’) of an ectomycorrhizal fungus Tricholoma matsutake in a Pinus densiflora forest. Fungal Ecol. 2015;15:18–28.

Carlile M, Watkinson S, Gooday G. The fungi. Second. London: Academic Press; 2001.

De la Varga H, Águeda B, Ágreda T, Martínez-Peña F, Parladé J, Pera J. Seasonal dynamics of Boletus edulis and Lactarius deliciosus extraradical mycelium in pine forests of Central Spain. Mycorrhiza. 2013;23(5):391–402.

Ohta A. A new medium for mycelial growth of mycorrhizal fungi. Trans Mycol Soc Japan. 1990;31(3):323–34.

Hamada M. Matsutake. Shizen. 1953;8(10):56–64. Japanese

Yamada A, Kanekawa S, Ohmasa M. Ectomycorrhiza formation of Tricholoma matsutake on Pinus densiflora. Mycoscience. 1999;40:193–8.

Fukasawa Y. Temperature effects on hyphal growth of wood-decay basidiomycetes isolated from Pinus densiflora deadwood. Mycoscience. 2018;59(3):259–62.

Dowson CG, Boddy L, Rayner ADM. Development and extension of mycelial cords in soil at different temperatures and moisture contents. Mycol Res. 1989;92(4): 383–91.

Sato T, Yaegashi K, Ishii S, Hirano T, Kajiwara S, Shishido K, et al. Transformation of the edible basidiomycete Lentinus edodes by restriction enzyme-mediated integration of plasmid DNA. Biosci Biotechnol Biochem. 1998;62(12): 2346–50.

Yamada A, Katsuya K. Mycorrhizal association of isolates from sporocarps and ectomycorrhizas with Pinus densiflora seedlings. Mycoscience. 1995;36(3):315–23.

Yamaguchi M, Narimatsu M, Fujita T, Kawai M, Kobayashi H, Ohta A, et al. A qPCR assay that specifically quantifies Tricholoma matsutake biomass in natural soil. Mycorrhiza. 2016;26(8):847–61.

R Development Core Team. R: A language and environment for statistical computing; 2015.

Kawai M, Abe S. Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. I. Effects of carbon and nitrogen sources in media on the vegetative growth of T. matsutake. Trans Mycol Soc Japan. 1976;17:159–67. Japanese

Vaario L, Yang X, Yamada A. Biogeography of the Japanese gourmet fungus, Tricholoma matsutake: A review of the distribution and functional ecology of matsutake. In: Tedersoo L, Editor. Biogeography of Mycorrhizal Symbiosis. Cham: Springer. 2017;319–44.

World Weather Online; 2019.
(Accessed 5 November 2019)

Lee W, Han S, Kim B, Shrestha B, Lee S, Ko C, et al. Proliferation of Tricholoma matsutake mycelial mats in pine forest using mass liquid inoculum. Mycobiology. 2007;35(2):54–61.

Akama K, Okabe H, Yamanaka T. Growth of ectomycorrhizal fungi on various culture media. Bull For For Prod Res Inst. 2008;7(4):165–81. Japanese

Todo C, Torigoe S. Studies on cultivation method of Tricholoma fulvocastaneum (I) The physiological characteristics. Bull Hyogo Prefect For For Technol Cent. 1996;49:1–4. Japanese

Jeon S-M, Ka K-H, Hong K-S. Mycelial growth and in vitro ectomycorrhizal synthesis on Pinus densiflora seedlings of Tricholoma bakamatsutake in Korea. Korean J Mycol. 2014;42(4):312– 21.

Omori H. The affect of weather conditions on the production volume of matsutake in Iwate prefecture. Bull Iwate Prefect For Technol Cent. 1997;7:67–72. Japanese