Treatment of Port Harcourt Refinery Effluent by a Bacterial Consortium Immobilized on Agro-based Bio Carriers

Main Article Content

G. C. Iheanacho
A. A. Ibiene
P. O. Okerentugba

Abstract

Discharge of poorly treated refinery wastewater has always been a major environmental challenge. Bacterial immobilization is key to the maintenance of biomass on a contaminated site. In this study, a mixed culture of three bacterial isolates from oil-polluted water: Pseudomonas aeruginosa (MN294989), Bacillus tequilensis (MN294990) and Micrococcus sp. immobilized on Groundnut Shell (GS), Melon Husk (MH) and Sugarcane Bagasse (SB) were employed in the bioremediation of Port Harcourt refinery wastewater. Surface area and pore size distribution of the agro-based bio carriers were suitable for bacteria adhesion. The bacterial isolates were screened for phenol, naphthalene and hydrocarbon utilization. Scanning Electron Microscopy (SEM) was used to ascertain the immobilization of the consortium on the agro-base carriers. A 15-days laboratory-scale treatment of refinery raw wastewater was compared in the immobilised and immobilized consortium. The agro-based residue immobilized consortium enhanced the reduction in BOD5, COD, oil and grease, phenol by 7%, 9%, 30% and 5% respectively compared to the free form of the consortium. This study underscores the role of immobilization in maintaining high bacterial biomass on contaminated site and possible improvement in bioremediation of refinery wastewater.

Keywords:
Immobilized consortium, bioremediation, refinery raw wastewater, bio carriers, phenol utilization.

Article Details

How to Cite
Iheanacho, G. C., Ibiene, A. A., & Okerentugba, P. O. (2019). Treatment of Port Harcourt Refinery Effluent by a Bacterial Consortium Immobilized on Agro-based Bio Carriers. Asian Journal of Biotechnology and Bioresource Technology, 5(4), 1-10. https://doi.org/10.9734/ajb2t/2019/v5i430069
Section
Original Research Article

References

Kadafa AA. Oil spillage and pollution in Nigeria: organizational management and institutional framework. Journal of Environ-mental Earth Science. 2012;2(4):38–51.

Osin OA, Yu T, Lin S. Oil refinery wastewater treatment in the Niger Delta, Nigeria: Current practices, challenges and recommendations. Environmental Science and Pollution Research. 2017;24:22730–22740.
Available:https://doi.org/10.1007/s11356-017-0009-z

Yu L, Han MHF. A review of treating oily wastewater. Arabian Journal of Chemistry. 2017;10:s1913–s1922. Available:https://doi.org/10.1016/j.arabjc.2013.07.020

Deka G, Devi A, Bhattacharyya GK. Impact of oil refinery wastes on water and soil quality: A case study. Environmental Science An Indian Journal. 2013;7(1): 3483–3507.
Available:https://doi.org/10.1039/ c3ee42518e

Ishak S, Malakahmad A, Isa MH. Refinery wastewater biological treatment: A short review. Journal of Scientific and Industrial Research. 2012;71(4):251–256.

Mahiuddin M, Fakhruddin ANM, Al-Mahin AM. Degradation of phenol via meta cleavage pathway by Pseudomonas fluorescens PU1. International Schorlarly Research Network. 2012;1-6.
Available:https://doi.org/10.5402/2012/741820

Poi G, Aburto-Medina A, Mok PC, Ball AS, Shahsavari E. Bioremediation of phenol-contaminated industrial wastewater using a bacterial consortium—from laboratory to field. Water, Air, and Soil Pollution. 2017; 228(3).
Available:https://doi.org/10.1007/s11270-017-3273-0

Bayat Z, Hassanshahian M, Cappello S. Immobilization of microbes for bioremedia-tion of crude oil polluted environments: A mini-review. The Open Microbiology Journal. 2015;9:48–54.
Available:https://doi.org/10.2174/1874285801509010048

Cláudia S, Martins S, Martins CM, Maria L, Guedes C, Santaella ST. Immobilization of microbial cells: A promising tool for treat-ment of toxic pollutants in industrial waste-water. African Journal of Biotechnology. 2013;12(28):4412–4418.

Available:https://doi.org/10.5897/ AJB12.2677

Liu H, Guo L, Liao S, Wang G. Reutilization of immobilized fungus Rhizopus sp. LG04 to reduce toxic chromate. 2012;(Iii):651–659.
Available:https://doi.org/10.1111/j.1365-2672.2012.05257.x

Ekwuabu CB, Chikere CB, Akaranta O. Effect of different nutrient amendments on eco-restoration of a crude oil polluted soil; 2016.
Available:https://doi.org/10.2118/183608-ms

Xue J, Wu Y, Liu Z, Li M, Sun X, Wang H, Liu B. Characteristic assessment of diesel-degrading bacteria immobilized on natural organic carriers in marine environment: The degradation activity and nutrient. Scientific Reports. 2017;7(1):1–9.
Available:https://doi.org/10.1038/s41598-017-08832-y

Velmurugan AM, Arunachalam C. Biore-mediation of phenol and naphthalene by Bacillus species and Brachybacterium species isolated from pharma soil sample. Current World Environment. 2009;4(2): 299–306. Available:https://doi.org/10.12944/cwe.4.2.06

Dzionek A, Wojcieszyńska D, Guzik U. Natural carriers in bioremediation: A review. Electronic Journal of Biotechno-logy. 2016;23:28–36.
Available:https://doi.org/10.1016/j.ejbt.2016.07.003

Lin M, Liu Y, Chen W, Wang H, Hu X. Use of bacteria-immobilized cotton fibers to absorb and degrade crudeoil. International Biodeterioration and Biodegradation. 2014; 88:8–12.
Available:https://doi.org/10.1016/j.ibiod.2013.11.015

Udawatte CHR, Sotheeswaran SWS. Immobilization of selected microbes at some selected solid supports for enhanced fermentation process. Fermentation Technology. 2015;04(01):1–4.
Available:https://doi.org/10.4172/2167-7972.1000115

Rehman A, Ilyas S. Isolation of phenol degrading bacteria from industrial effluents and their potential use in waste water treatment. Proceedings of Pakistan Congress of Zoology. 2008;28:1–10.

Sridevi V, Lakshmi MVVC, Manasa M, Sravani M. Metabolic pathways for the biodegradation of phenol. International Journal of Engineering Science and Advanced Technology. 2012;3:695–705.

Obuekwe CO, Al-Muttawa EM. Self-immobilized bacterial cultures with potential for application as ready-to-use seeds for petroleum bioremediation. Biotechnology Letters. 2001;23(13):1025–1032. Available:https://doi.org/10.1023/A:1010544320118

Baharvand S, Reza M, Daneshvar M. Impact assessment of treated wastewater on the physicochemical variables of the environment: A case of Kermanshah wastewater treatment plant in Iran. Environmental Systems Research; 2019.
Available:https://doi.org/10.1186/s40068-019-0146-0

Carducci A, Verani M. Effects of bacterial, chemical, physical and meteorological variables on virus removal by a wastewater treatment plant effects of bacterial, chemical, physical and meteorological variables on virus removal by a wastewater treatment plant. Food and Environmental Virology. 2013;5:59-76.
Available:https://doi.org/10.1007/s12560-013-9105-5

Ajao AT, Yakubu SE, Umoh VJ, Ameh JB. Bioremediation of refinery wastewater using immobilised Burkholderia cepacia and Corynebacterium sp and their transconjugants. Journal of Xenobiotics. 2013;3(1):4.
Available:https://doi.org/10.4081/xeno.2013.e4

Nuñal SN, Santander-De Leon SMS, Bacolod E, Koyama J, Uno S, Hidaka M, Maeda H. Bioremediation of heavily oil-polluted seawater by a bacterial consortium immobilized in cocopeat and rice hull powder. Biocontrol Science. 2014;19(1): 11–22.
Available:https://doi.org/10.4265/bio.19.11