Data on the Draft Genome Sequence of Bacillus sp. Strain AN2 Isolated from Agricultural Soil in Brazil

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Andressa Sbano
Juliana B. Succar
João Victor R. Ferreira
Lidiane C. Berbert
Vinicius R. Flores
Janaína J. V. Cavalcante
Rodolpho M. Albano
Ida Carolina N. Direito
Alexander M. Cardoso


Aims: This research aims to report the genome sequence of the Bacillus sp. strain AN2, isolated from agricultural soil from Rio de Janeiro, Brazil.

Place and Duration of Study: Laboratory of Environmental Biotechnology, Western Rio Janeiro State University in Brazil, between January 2019 and December 2019.

Methodology: High-quality genomic DNA was extracted using a GenElute Bacterial Genomic DNA kit. The Nextera XT DNA Library Prep Kit was used for genomic library construction. Paired-end sequence reads were generated by an Illumina MiSeq instrument with the 600 cycles MiSeq Reagent Kit v3. Sequence data were assembled with A5-MiSeq pipeline software and the contigs were annotated by Rapid Annotation using Subsystems Technology (RAST).

Results: The obtained genome sequence of Bacillus AN2 included 21 contigs with a calculated size of 3,681,081 bp in length. The G + C content for the draft genome is 41.4%. A total of 3824 coding sequences (CDS) were predicted and encoded at least 88 tRNAs.

Biofertilizers, biotechnology, inoculants, soil microbiota.

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How to Cite
Sbano, A., Succar, J. B., Ferreira, J. V. R., Berbert, L. C., Flores, V. R., Cavalcante, J. J. V., Albano, R. M., Direito, I. C. N., & Cardoso, A. M. (2020). Data on the Draft Genome Sequence of Bacillus sp. Strain AN2 Isolated from Agricultural Soil in Brazil. Asian Journal of Biotechnology and Bioresource Technology, 6(1), 11-13.
Data Article


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