Preliminary Antibacterial Screening of the Whole Stem of Rothmannia whitfieldii
Asian Journal of Biotechnology and Bioresource Technology, Volume 8, Issue 4,
Page 58-67
DOI:
10.9734/ajb2t/2022/v8i4167
Abstract
Aim: To assess the antibacterial activity of the crude ethanolic and water extracts of the stem of Rothmannia whitfieldii on clinically isolated and typed pathogenic bacterial strains.
Study Design: This experiment was performed in duplicates under aseptic condition.
Place and Duration of Study: Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria between April 2005 to August, 2005.
Methodology: Whole dried stem specimen of Rothmannia whitfieldii was extracted by maceration and screened for antibacterial activity using agar well-diffusion technique. Five typed bacterial strains namely, Staphylococcus aureus (ATCC 12600), Pseudomonas aeruginosa (ATCC 10145), Escherichia coli (ATCC 11775), Salmonella kintambo (SSRL 113) and Bacillus subtilis (ATCC 6051), and six locally isolated clinical strains (Escherichia coli, Salmonella typhi, P. aeruginosa, S. aureus and two Proteus species designated as Proteus I and Proteus II), were tested for susceptibility to the plant extract.
Results: Phytochemical analysis revealed the presence of flavonoids, saponnins, tannins, carbohydrates and steroidal aglycone. Alkaloid was present in trace amount only in the ethanolic extract. The ethanolic extract exhibited a wider spectrum of activity, being effective against 81.8% of the test organisms compared with the cold-water extract (54.5%) or hot water extracts (27.3%). The minimum inhibitory concentrations (MICs) varied not only with the test organisms but also with the various extracts of the plant.
Conclusion: All the extracts had bacteriostatic activity on the susceptible organisms and none was bactericidal. The screening test results authenticate the folkloric claims that Rothmannia whitfieldii is medicinal against gastrointestinal diseases and aid in wound healing.
- Rothmannia whitfieldii
- preliminary screening
- antibacterial activity
- aqueous extract
- ethanolic extract and whole stem
How to Cite
References
Nasrullah, Suliman, Rahman K, Ikram M, Nisar M, Khan I. Screening of Antibacterial Activity of Medicinal Plants. International Journal of Pharmaceutical Science Review and Research. 2012; 14(2):25‐29.
Pai-Wei S, Cheng-Hong Y, Jyh-Ferng Y, Pei-Yu S, Li-Yeh C. Antibacterial Activities and Antibacterial Mechanism of Polygonum cuspidatum Extracts against Nosocomial Drug-Resistant Pathogens. Molecules. 2015; 20: 11119-11130.
DOI: 10.3390/molecules200611119.
Rahman K, Nisar M, Ullah Jan A, Suliman M, Iqbal A, Ahmad A, Ghaffar R. Antibacterial Activity of Important Medicinal Plants on Human Pathogenic Bacteria. International Journal of Agronomy and Agricultural Research (IJAAR). 2015; 6(6):106-111.
Manandhar S, Luitel S, Dahal RK. In vitro Antimicrobial Activity of Some Medicinal Plants against Human Pathogenic Bacteria. Journal of Tropical Medicine. 2019;article 1895340.
Availaable:https://doi.org/10.1155/2019/1895340.
Bajpai M, Pande A, Tewari SK, Prakash D. Phenolic Contents and Antioxidant Activity of Some Food and Medicinal Plants. International Journal of Food Sciences and Nutrition. 2005;56(4): 287-291.
Wojdylo A, Oszmianski J, and Czemerys R. Antioxidant Activity and Phenolic Compounds in 32 Selected Herbs. Food Chemistry. 2007;105:940-949.
Olanrewaju IF, Raphael C, Mordi RC, John Bull O, Echeme JO. Antibacterial, Antifungal and Anti-tubercular Activities of Chloroform Fraction of the Leaf Extract of Irvingia Gabonensis (African Bush Mango). Anti-Infective Agents. 2019; 17 (1).
Malik F, Hussain S, Mirza T, Hameed A, Ahmad S, Riaz R, Shah PA, Usmanghani K. Screening for Antimicrobial Activity of thirty-three Medicinal Plants used in the Traditional System of Medicine in Pakistan. Journal of Medicinal Plant Research. 2011;5(14):3052-3060.
Walter C, Shinwari ZK, Afzal I, Malik RN. Antibacterial Activity in Herbal Products Used In Pakistan. Pakistan Journal of Botany. 2011;43:155-162.
Jansen PCM. Rothmannia whitfieldii (Lindl.) Dandy. In: Jansen PCM, Cardon D, Eds., Plant Resources of Tropical Africa, PROTA, Wageningen: 2005;1-2.
Available:http://www.prota4u.org
Osabohien E, Otutu JO. Extraction and Utilization of Natural Dyestuffs from the Bark of Whistling Pine and the Root of African Peach. British Journal of Applied Science & Technology, 2014;4:2921- 2930. Available:https://doi.org/10.9734/BJAST/2014/8554
Nnorom OO, Onuegbu GC. Authentication of Rothmannia whitfieldii Dye Extract with FTIR Spectroscopy. Journal of Textile Science and Technology. 2019;5:38- 47.
Available: https://doi.org/10.4236/jtst. 2019.52004.
Harbone JB. The Flavonoids: Advances in Research Since 1986. Chapman and Hall Ltd., London.1994;330.
National Committee for Clinical Laboratory Standards (NCCLS). Methods for Dilution in Antimicrobial Susceptibility Tests: Approved Standard M2-A5. National Committee for Clinical Laboratory Standards: Villanova, P.A; 1993.
Okeke MI, Iroegbu CU, Eze EN, Okoli AS, Esimone CO. Evaluation of Extracts of the Root of Landolphia owerrience for Antibacterial Activity. Journal of Ethnopharmacoogy. 2001;78(2-3): 119-127.
Ibrahim, M.B., M.O. Owonubi, and J.A. Onaolapo. Antimicrobial Effects of Extracts of Leaf, Stem and Root Bark of Anogiessus leicarpus on Staphylococcus aureus NCTC 8198, Escherichia coli NCTC 10418, and Proteus vulgaris NCTC 4636. Journal of Pharmaceutical Research and Development 1007; 2:20-26.
Levan M, Vanden Berghe DA, Marten T, Vilientmick A, Lomweas EC. Screening of Higher Plants for Biological Activity. Planta Medica. 1979;36:311-312.
Awosan EA, Lawal IO, Ajekigbe JM, Borokini TI. Antimicrobial potential of Rothmannia longiflora Salisb and Canna indica Linn extracts against selected strains of fungi and bacteria. African Journal of Microbiology Research, 2014;8 (24):2376-2380.
DOI:10.5897/AJMR2013.6360
Van HT, Nguyen TT, Thi Ton TH, Thi Dinh TK, Le VS, Pham TV, Tran GB. Phytochemical composition and antibacterial activity of ethanolic extract from trunk of Rothmannia wittii (Craib) Bremek. (Rubiaceae) newly found in Vietnam. Journal of Biotechnology, Computational Biology and Bionanotechnology. 2020;101(4):301–307
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