Ammonia Pre-Treated Cotton Stalks for Bioethanol Production using Saccharomyces cerevisiae CP11
Asian Journal of Biotechnology and Bioresource Technology,
Page 81-87
DOI:
10.9734/ajb2t/2022/v8i4169
Abstract
Cotton stalks are good raw material for bioethanol production due to its availability throughout the world, abundance, high carbohydrate content and not involved in any food chain. Due to recalcitrant nature of cotton stalks pre-treatment, hydrolysis were not effective. In the present study pre-treatment with ammonia at room temperature for 1 week period and 121oC for 60min were attempted and it is compared with the standard 0.2M NaOH treatment. 1.5% ammonia pre-treatment at room temperature for 1 week found to remove 86% of lignin and subsequently undergone 75.92% acid hydrolysis. The acid hydrolysate obtained consists less lignin and furfurals and fermented to 5.75% ethanol with 91% fermentation efficiency with saccharomyces cerevisiae. Hence dilute ammonia pre-treatment for a week was found to be effective for bioethanol production using cotton stalks.
Keywords:
- Ammonia
- pre-treatment
- cotton stalk
- bioethanol
- yeast
How to Cite
Goutham, Y. S., Pasha, S. M., Naveen, M., & Pasha, C. (2022). Ammonia Pre-Treated Cotton Stalks for Bioethanol Production using Saccharomyces cerevisiae CP11. Asian Journal of Biotechnology and Bioresource Technology, 8(4), 81-87. https://doi.org/10.9734/ajb2t/2022/v8i4169
References
McKendry, P. Energy production from biomass (part 1): overview of biomass. Bioresour Technol. 2002;83:37-46.
Dien BS, Iten LB, Bothast RJ. Conversion of corn fiber to ethanol by recombinant E.coli strain FBR3. J Ind Microbiol. 1999;22:575-581.
Haykir NI, Bakir U. Ionic liquid pretreatment allows utilization of high substrate loadings in enzymatic hydrolysis of biomass to produce ethanol from cotton stalks. Ind Crop Prod. 2013;51:408–414.
Kaur U, Oberoi HS, Bhargav VK, Sharma-Shivappa R, Dhaliwal SS. Ethanol production from alkali- and ozonetreated cotton stalks using thermotolerant Pichia kudriavzevii HOP-1. Ind Crop Prod. 2012;37:219–226.
Binod P, Kuttiraja M, Archana M, Janu KU, Sindhu R, Sukumaran RK, Pandey A. High temperature pretreatment and hydrolysis of cotton stalk for producing sugars for bioethanol production. Fuel. 2012;92:340–345.
Meixia Wang, Dayun Zhou, Yanqin Wang, Shoujun Wei, Weihua Yang, Meng Kuang, Lei Ma, Dan Fang, Shuangjiao Xu, Shuang-kui Du. Bioethanol production from cotton stalk: A comparative study of various pretreatments. Fuel. 2016; 184:527-532.
McIntosh S, Vancov T, Palmer J, Morris S. Ethanol production from cotton gin trash using optimised dilute acid pretreatment and whole slurry fermentation processes. Bioresour Technol. 2014;173:42–51.
Du SK, Su X, Yang W, Wang Y, Kuang M, Ma L, Fang D, Zhou D. Enzymatic saccharification of high pressure assist alkali pretreated cotton stalk and structural characterization. Carbohyd Polym. 2016; 140:279–286.
Du SK, Zhu X, Wang H, Zhou D, Yang W, Xu H. High pressure assist-alkali pretreatment of cotton stalk and physiochemical characterization of biomass. Bioresour Technol. 2013; 148:494–500.
Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne JA. comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol. 2007;98:3000–3011.
Chang VS, Holtzapple MT. Fundamental factors affecting biomass enzymatic reactivity. Appl Biochem Biotechnol. 2000;84–6:5–37.
Chen LJ, Xu YL, Bai FW, Anderson WA, Murray MY. Observed quasi-steady kinetics of yeast cell growth and ethanol formation under very high gravity fermentation condition. Biotechnol Bioprocess Eng. 2005;10:115-121.
Kim SB, Lee SJ, Lee JH, Jung YR, Thapa LP, Kim JS, et al. Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia. Biotechnol Biofuels. 2013;6(1):109.
Shi J, Sharma-Shivappa RR, Chinn M, Howell N. Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioenergy. 2009;33(1):88–96.
Rehman MSU, Kim I, Kim KH, Han JI. Optimization of sono-assisted dilute sulfuric acid process for simultaneous pretreatment and saccharification of rice straw. Int J Environ Sci Technol. 2014;11(2):543–50.
Singh R, Shukla A, Tiwari S, Srivastava M. A review on delignification of lignocellulosic biomass for enhancement of ethanol production potential. Renew Sust Energ Rev. 2014;32:713– 728.
Alvira P, Tomas-Pejo E, Ballesteros M, Negro MJ. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol. 2010;101(13):4851–61.
Kapoor M, Raj T, Vijayaraj M, Chopra A, Guptaa RP, Tuli DK, et al. Structural features of dilute acid, steam exploded, and alkali pretreated mustard stalk and their impact on enzymatic hydrolysis. Carbohyd Polym. 2015;124:265–73.
Hsu, T. A. Pretreatment of biomass. In: C. E. Wyman (ed.). Handbook on Bioethanol: Production and Utilization. Taylor & Francis, Washington DC, USA. 1996;179-195.
Bahcegul E, Toraman HE, Ozkan N, Bakir U. Evaluation of alkaline pretreatment temperature on a multi-product basis for the co-production of glucose and hemicellulose based films from lignocellulosic biomass. Bioresour Technol. 2012;103:440–445.
Vani S, Binod P, Kuttiraja M, Sindhu R, Sandhya SV, Preeti VE, Sukumaran RK, Pandey A. Energy requirement for alkali assisted microwave and high pressure reactor pretreatments of cotton plant residue and its hydrolysis for fermentable sugar production for biofuel application. Bioresour Technol. 2012;112:300–307.
TAPPI. Technical Association of Pulp and Paper Institute, Atlanta, Georgia, USA. 1992.
Mirza Zaheer Baig, Smita M Dharmadhikari. Bioethanol Production from Cotton Stalk Hydrolysate using Immobilized Co culture of Saccharomyces cerevisiae and Pachysolen tannophilus. Int J Curr Microbiol App Sci. 2016;5(12): 389-397.
Miller GL. Use of dinitro salicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31:426–428.
Singleton VL, Rossi JA. Colorimetric of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult.1965;16:144-158.
Despoina Chilari, Konstantinos Dimos, Georgia Georgoula, Thomas Paschos, Diomi Mamma, Argiro Louloudi, Nikolaos Papayannakos, Dimitris Kekos. Bioethanol Production from Alkali-Treated Cotton Stalks at High Solids Loading Applying Non - isothermal Simultaneous Saccharification and Fermentation. Waste Biomass Valor. 2017;8:1919–1929.
Shahzad K, Sohail M, Hamid A. Green ethanol production from cotton stalk. IOP Conf Series: Earth and Environmental Science. 2019;257:012025.
Praveen Kumar Keshav, Chandrashekhar Banoth, Srinivas Naik, Kethavath, Bhima Bhukya. Lignocellulosic ethanol production from cotton stalk: an overview on pretreatment, saccharification and fermentation methods for improved bioconversion process. Biomass Conversion and Biorefinery. 2021;1-18.
Vazquez M, Oliva M, Tellez-Luis SJ, Ramírez JA. Hydrolysis of sorghum straw using phosphoric acid: evaluation of furfural production. Bioresour Technol. 2007;98:3053–3060.
Wendhausen R, Fregonesi A, Moran PJS, Joekes I, Rodrigues JA, Tonella E, Althoff K. Continuous fermentation of sugar cane syrup using immobilized yeast cells. J Biosci Bioeng. 2001;9(1):48-52.
Dien BS, Iten LB, Bothast RJ. Conversion of corn fiber to ethanol by recombinant E.coli strain FBR3. J Ind Microbiol. 1999;22:575-581.
Haykir NI, Bakir U. Ionic liquid pretreatment allows utilization of high substrate loadings in enzymatic hydrolysis of biomass to produce ethanol from cotton stalks. Ind Crop Prod. 2013;51:408–414.
Kaur U, Oberoi HS, Bhargav VK, Sharma-Shivappa R, Dhaliwal SS. Ethanol production from alkali- and ozonetreated cotton stalks using thermotolerant Pichia kudriavzevii HOP-1. Ind Crop Prod. 2012;37:219–226.
Binod P, Kuttiraja M, Archana M, Janu KU, Sindhu R, Sukumaran RK, Pandey A. High temperature pretreatment and hydrolysis of cotton stalk for producing sugars for bioethanol production. Fuel. 2012;92:340–345.
Meixia Wang, Dayun Zhou, Yanqin Wang, Shoujun Wei, Weihua Yang, Meng Kuang, Lei Ma, Dan Fang, Shuangjiao Xu, Shuang-kui Du. Bioethanol production from cotton stalk: A comparative study of various pretreatments. Fuel. 2016; 184:527-532.
McIntosh S, Vancov T, Palmer J, Morris S. Ethanol production from cotton gin trash using optimised dilute acid pretreatment and whole slurry fermentation processes. Bioresour Technol. 2014;173:42–51.
Du SK, Su X, Yang W, Wang Y, Kuang M, Ma L, Fang D, Zhou D. Enzymatic saccharification of high pressure assist alkali pretreated cotton stalk and structural characterization. Carbohyd Polym. 2016; 140:279–286.
Du SK, Zhu X, Wang H, Zhou D, Yang W, Xu H. High pressure assist-alkali pretreatment of cotton stalk and physiochemical characterization of biomass. Bioresour Technol. 2013; 148:494–500.
Silverstein RA, Chen Y, Sharma-Shivappa RR, Boyette MD, Osborne JA. comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresour Technol. 2007;98:3000–3011.
Chang VS, Holtzapple MT. Fundamental factors affecting biomass enzymatic reactivity. Appl Biochem Biotechnol. 2000;84–6:5–37.
Chen LJ, Xu YL, Bai FW, Anderson WA, Murray MY. Observed quasi-steady kinetics of yeast cell growth and ethanol formation under very high gravity fermentation condition. Biotechnol Bioprocess Eng. 2005;10:115-121.
Kim SB, Lee SJ, Lee JH, Jung YR, Thapa LP, Kim JS, et al. Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia. Biotechnol Biofuels. 2013;6(1):109.
Shi J, Sharma-Shivappa RR, Chinn M, Howell N. Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioenergy. 2009;33(1):88–96.
Rehman MSU, Kim I, Kim KH, Han JI. Optimization of sono-assisted dilute sulfuric acid process for simultaneous pretreatment and saccharification of rice straw. Int J Environ Sci Technol. 2014;11(2):543–50.
Singh R, Shukla A, Tiwari S, Srivastava M. A review on delignification of lignocellulosic biomass for enhancement of ethanol production potential. Renew Sust Energ Rev. 2014;32:713– 728.
Alvira P, Tomas-Pejo E, Ballesteros M, Negro MJ. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol. 2010;101(13):4851–61.
Kapoor M, Raj T, Vijayaraj M, Chopra A, Guptaa RP, Tuli DK, et al. Structural features of dilute acid, steam exploded, and alkali pretreated mustard stalk and their impact on enzymatic hydrolysis. Carbohyd Polym. 2015;124:265–73.
Hsu, T. A. Pretreatment of biomass. In: C. E. Wyman (ed.). Handbook on Bioethanol: Production and Utilization. Taylor & Francis, Washington DC, USA. 1996;179-195.
Bahcegul E, Toraman HE, Ozkan N, Bakir U. Evaluation of alkaline pretreatment temperature on a multi-product basis for the co-production of glucose and hemicellulose based films from lignocellulosic biomass. Bioresour Technol. 2012;103:440–445.
Vani S, Binod P, Kuttiraja M, Sindhu R, Sandhya SV, Preeti VE, Sukumaran RK, Pandey A. Energy requirement for alkali assisted microwave and high pressure reactor pretreatments of cotton plant residue and its hydrolysis for fermentable sugar production for biofuel application. Bioresour Technol. 2012;112:300–307.
TAPPI. Technical Association of Pulp and Paper Institute, Atlanta, Georgia, USA. 1992.
Mirza Zaheer Baig, Smita M Dharmadhikari. Bioethanol Production from Cotton Stalk Hydrolysate using Immobilized Co culture of Saccharomyces cerevisiae and Pachysolen tannophilus. Int J Curr Microbiol App Sci. 2016;5(12): 389-397.
Miller GL. Use of dinitro salicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31:426–428.
Singleton VL, Rossi JA. Colorimetric of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult.1965;16:144-158.
Despoina Chilari, Konstantinos Dimos, Georgia Georgoula, Thomas Paschos, Diomi Mamma, Argiro Louloudi, Nikolaos Papayannakos, Dimitris Kekos. Bioethanol Production from Alkali-Treated Cotton Stalks at High Solids Loading Applying Non - isothermal Simultaneous Saccharification and Fermentation. Waste Biomass Valor. 2017;8:1919–1929.
Shahzad K, Sohail M, Hamid A. Green ethanol production from cotton stalk. IOP Conf Series: Earth and Environmental Science. 2019;257:012025.
Praveen Kumar Keshav, Chandrashekhar Banoth, Srinivas Naik, Kethavath, Bhima Bhukya. Lignocellulosic ethanol production from cotton stalk: an overview on pretreatment, saccharification and fermentation methods for improved bioconversion process. Biomass Conversion and Biorefinery. 2021;1-18.
Vazquez M, Oliva M, Tellez-Luis SJ, Ramírez JA. Hydrolysis of sorghum straw using phosphoric acid: evaluation of furfural production. Bioresour Technol. 2007;98:3053–3060.
Wendhausen R, Fregonesi A, Moran PJS, Joekes I, Rodrigues JA, Tonella E, Althoff K. Continuous fermentation of sugar cane syrup using immobilized yeast cells. J Biosci Bioeng. 2001;9(1):48-52.
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