Assessment of Airborne Extended Spectrum β-Lactamase Producing Enterobacteriaceae Isolates from Motor Parks in Benin City, Nigeria

Cyprian E. Oshoma *

Department of Microbiology, Faculty of Life Sciences, University of Benin, P.M.B. 1154, Benin City, Nigeria.

Frank M. Akro

Department of Microbiology, Faculty of Life Sciences, University of Benin, P.M.B. 1154, Benin City, Nigeria.

Patrick O. Akpoghelie

Department of Food Science and technology, Faculty of Science, Delta State University of Science and Technology, Ozoro, Nigeria.

Osayi B. Isichei-Ukah

Department of Microbiology, Faculty of Life Sciences, University of Benin, P.M.B. 1154, Benin City, Nigeria.

Solomon E. Omonigho

Department of Microbiology, Faculty of Life Sciences, University of Benin, P.M.B. 1154, Benin City, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Aim: The study was aimed at assessing airborne bacteria and extended spectrum β-lactamase producing Enterobacteriaceae isolated from the motor parks in Benin City.

Methodology: Airborne bacteria samples were collected in the motor parks (Central, Ugbowo, Agip, 19th Street, and Ekosodin Park), in Benin City using the settled plate method on Nutrient and MacConkey agar. Temperature, relative humidity, and airborne bacteria of the different parks were determined. The bacterial isolates were identified using phenotypic method. The antibiotic susceptibility profile was investigated by the disc diffusion method. The Enterobacteriaceae isolates were screened for Extended-spectrum β-lactamase (ESBL) production potentials. Virulence factor analysis and detection of ESBL genes (TEM, CTX-M, SHV, and OXA) were carried out on the selected ESBL producers.

Results: The relative humidity recorded at the parks at the point of agar plate exposure ranged from 82  ̶  90%. The airborne heterotrophic bacterial and coliform counts from the motor parks ranged from 9.50±0.63 × 102  ̶  1.18±0.02 ×103 cfu/m3 and 2,48±0.41×102  ̶  3.30±0.41×102 cfu/m3 respectively. The identified Enterobacteriaceae were Citrobacter freundii, Serratia marcescens, Citrobacter koseri, Klebsiella pneumoniae, Morganella morganii, Klebsiella oxytoca, Shigella dysenteriae, Proteus mirabilis, Escherichia coli, Enterobacter cloacae and Citrobacter diversus. The isolate with highest frequency of occurrence was E. coli with values of 30.48 %. The isolate K. pneumoniae had the highest MAR index of 0.8. The isolate S. marcescens had the highest virulent factor of 60% and possessed all the ESBL genes detected. 

Conclusion: Strong infection control measures should be put in place because more ESBL-producing Enterobacteriaceae have emerged in the surroundings of specific parks in Benin City.

Keywords: Enterobacteriaceae, motor park, airborne, temperature, ESBL


How to Cite

E. Oshoma, Cyprian, Frank M. Akro, Patrick O. Akpoghelie, Osayi B. Isichei-Ukah, and Solomon E. Omonigho. 2024. “Assessment of Airborne Extended Spectrum β-Lactamase Producing Enterobacteriaceae Isolates from Motor Parks in Benin City, Nigeria”. Asian Journal of Biotechnology and Bioresource Technology 10 (2):72-84. https://doi.org/10.9734/ajb2t/2024/v10i2206.

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References

Salami JT, Sawyerr HO, Dada AA. Assessment of Air Quality in major motor parks in Ilorin Metropolis, Kwara State, Nigeria Int J Health Sci Res. 2020;10(10); 86 -95.

Ahmed YA. Menace of illegal motor parks in Nigerian urban environment: example

from Ilorin City. J Res Develop. 2016;3(1): 23-33.

Dick AA, Wekhe C. Microbial indoor air quality in a secondary school in Port Harcourt City, Rivers State, Nigeria. J Appl Sci. Environ Manage. 2020;24(7):1289-1292.

Yoo K, Lee TK, Choi EJ. Molecular approaches for the detection and monitoring of microbial communities in bioaerosols: A review. J Environ Sci. 2017;51: 234–247.

Willey JM., Sherwood LM, Woolverton CJ. Prescott’s Microbiology 9th edition. McGraw Hill, New York. 2014;1139.

Eghomwanre AF, Oguntoke O, Taiwo AM, Sam-Wobo SO, Enagbonma BJ. Assessment of airborne bacteria in residential buildings in Benin City, Nigeria. Ife J Sci. 2023;25(1):128 – 136.

Barberán A, Ladau J, Leff. Continental-scale distributions of dust associated bacteria and fungi. J Proc Nat Acad Sci. 2015;112:5756–5761.

Ohagim PI, Ikon GM, Matthew PC, Ohagim GA. Microbiological assessment of indoor air in public toilets across selected motor parks in Owerri Metropolis, Nigeria. J Microbiol Exp. 2017;5(6):14‒12.

Morris C, Sands D, Bardin M, Jaenicke R, Vogel B, Leyronas C, Ariya P, Psenner R. Microbiology and atmospheric processes: research challenges concerning the impact of airborne micro-organisms on the atmosphere and climate. Biogeoscience, 2011;8(1):17-25

Ekundayo OK, Onifade AK. Detection of beta-lactamase in ampicillin resisstance enterobacteriaceae isolated from human stool in Ekiti State, Nigeria. International Journal of Current Microbiology and Applied Sciences. 2020;9(4):2514-2524.

Paterson DL, Bonomo RA. Extended-Spectrum Beta-lactamases: a clinical update. Clinical Microbiology Review, 2005;18(4):657–86.

Paterson DL. Resistance in gram-negative Bacteria: Enterobacteriaceae. American Journal of Infection Control. 2006;119(6): 20–28.

Augustowska M, Dutkiewicz J. Variability of airborne microflora in a hospital ward within a period of one year. Journal of Annals of Agricultural and Environmental Medicine. 2006;13:99 –106.

Andualem Z, Gizaw Z, Bogale L, Dagne H. Indoor bacterial load and its correlation to physical indoor air quality parameters in public primary schools Multidisciplinary Respiratory Medicine 2019;14:2. Available:https://doi.org/10.1186/s40248-018-0167-y

Holt JG, Kreig NR, Sneath PHA, Staley JT, Williams ST. Bergey's manual of determinative bacteriology. 9th ed. MD: Williams & Wilkins, Baltimore; 1994.

Cheesbrough M. Microbiological test: District Laboratory practice in Tropical Countries. Editors: Cremer. A. and Evan, G. Cambridge University Press, Cambridge, Great British, U.K. 2000; 1-226.

Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S. Selection of chromosomal DNA libraries using a multiplex CRISPR system. Elife 3; 2014.

Edward EA, El Shehawy MR, Abouelfetouh A, Aboulmagd E. Prevalence of different virulence factors and their association with antimicrobial resistance among Pseudomonas aeruginosa clinical isolates from Egypt. BMC Microbiology. 2023;23:161. Available:https://doi.org/10.1186/s12866-023-02897-8

Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Volume. 26th edition., CLSI supplement M100S. Clinical and Laboratory Standards Institute, Wayne; 2016.

Odonkor ST, Addo KK. Prevalence of Multidrug-Resistant Escherichia coli Isolated from Drinking Water Sources International Journal of Microbiology Article ID 7204013, 2018;7. Available:https://doi.org/10.1155/2018/7204013

Livermoore DM, Paterson DL. Pocket guide to extended spectrum β-lactamases in resistance. Journal of Antimicrobial Chemotherapy. 2006;58:231-237.

Ahmed AB, Omar AO, Asghar AH, Elhassan MM. Prevalence of TEM, SHV and CTX M genes in Escherichia coli and Klebsiella spp. urinary isolates from Sudan with confirmed ESBL phenotype. Life Science Journal. 2013;10:191 195

Bali EB, Acik L, Sultan N. Phenotypic and molecular characterization of SHV, TEM, CTX-M and extended-spectrum beta-lactamase produced by Escherichia coli, Acinetobacter baumannii and Klebsiella isolates in a Turkish hospital. African Journal of Microbiological Research. 2010;4(8):650–654.

Colom K, Pérez J, Alonso R, Fernández Aranguiz A, Lariño E, Cisterna R. Simple and reliable multiplex PCR assay for detection of blaTEM, bla (SHV) and blaOXA 1 genes in Enterobacteriaceae. FEMS Microbiology Letters. 2003;223:147 151.

Sompolinsky D, Nitzan Y, Tetry S, Wolk M, Vulikh I, Kerrn MB, Katcoff DJ. Integron-mediated ESBL resistance in rare serotypes of Escherichia coli causing infections in an elderly population of Israel. Journal of Antimicrobial Chemotherapy. 2005;55(1):119–122.

Daam KC, Samuel DA, Nwokoro U, Waziri H, Onyedibe K, Okolo M, Edmund B, Olayinka A, Zanyu ED. Detection of CTX-M and SHV genes in Extended Spectrum Beta-Lactamase producing Klebsiella pneumoniae and Pseudomonas aeruginosa in a Tertiary Hospital in North-central Nigeria. Nigerian Medical Journal. 2023;64(2):196-204.

Mbim EN, Mboto CI, Edet UO. Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from two hospital environments in Calabar metropolis, Nigeria. Asian Journal of Medicine and Health. 2016; 1(1):1-11.

Nageen Y, Asemoloye MD, Põlme S, Wang X, Shihan Xu, S., Ramteke, P.W. and Pecoraro, P. Analysis of culturable airborne fungi in outdoor environments in Tianjin, China BMC Microbiology. 2021; 21:134.

Available:https://doi.org/10.1186/s12866-021-02205-2

Tang WJ. The effect of environmental parameters on the survival of airborne infectious agents Journal of Royal Society Interface. 2009;6,:S737–S746. DOI: 10.1098 /rsif.2009.0227.focus

Ruiz-Gil T, Acuna JJ, Fujiyoshi S, Tanaka D, Noda J, Maruyama F, Jorquera MA. Airborne bacterial communities of outdoor environments and their associated influencing factors Environment International. 2020;145:106156.

Quintana AR, Seseña S, Garzón A, Arias R. Factors Affecting Levels of Airborne Bacteria in Dairy Farms: A Review Animals 2020;10:526; doi:10.3390/ani10030526

Atalay YA, Mengistie E, Tolcha A, Birhan B, Asmare G, Gebeyehu NA and Gelaw KA. Indoor air bacterial load and antibiotic susceptibility pattern of isolates at Adare General Hospital in Hawassa, Ethiopia. Frontiers in Public Health. 2023;11: 1194850. DOI: 10.3389/fpubh.2023.1194850

Mulisa G, Selassie LG, Jarso G, Shiferew T, Zewdu A. Prevalence of extended Spectrum Beta-lactamase producing Enterobacteriaceae: A cross sectional study at Adama Hospital, Adama, Ethiopia. Journal of Emerging Infectious Diseases. 2016;1(1):1–6.

Aniebo CM, Stanley HO, Onwukwe CD. Assessment of the Indoor Air Quality of Majors’ Biological Laboratories in Ofrima Complex, University of Port-Harcourt, Nigeria. Journal of Petroleum & Environmental Biotechnology. 2016;7:285-295

Liu Y, Chen X, Luifu J, Zhao J, He X. Xie T. Extended-spectrum β-lactamase-producing Enterobacteriaceae from ready-to-eat foods: Genetic diversity and antibiotic susceptibility Food Science and Nutrition. 2023;11:5565–5572.

DOI: 10.1002/fsn3.3513

Agbagwa EO, Nwechem D. Public health significance of microorganisms associated with public restrooms in University of Port Harcourt. Scientia Africana. 2010;9:126– 132.

Ahmed MFE, Ramadan H, Seinige D, Kehrenberg C, El-Wahab AA, Volkmann N, Kemper N, Schulz J. Occurrence of extended-spectrum beta-lactamase-producing Enterobacteriaceae, microbial loads, and endotoxin levels in dust from laying hen houses in Egypt. BMC Veterinary Research 2020;16:301 Available:https://doi.org/10.1186/s12917-020-02510-4.

Andy IE, Okpo EA. Plasmid profile analysis and curing of multidrug resistant

Bacteria isolated from Hospitals waste dump site in Calabar Metropolis, Nigeria, European Journal of Pharmaceutical and Medical Research. 2019;6(5):54-61.

Datok, DW; Ishaleku, D; Tsaku, PA; Agya, EO; Adoga, MP Multidrug resistance to commonly prescribed antibiotics in Escherichia coli isolated from barbecued beef (Suya) sold in a Nigerian City. Pan Afri. Med. J. 2021;39(50). DOI: 10.11604/pamj.2021.39.50.25502

Chaudhary MK, Jadhav I, Banjara MR. Molecular detection of plasmid mediated blaTEM, blaCTX−M, and blaSHV genes in Extended Spectrum β-Lactamase (ESBL) Escherichia coli from clinical samples. Annals of Clinical Microbiology and Antimicrobials. 2023;22:33. Available:https://doi.org/10.1186/s12941-023-00584-0

Ogbolu DO, Daini OA, Ogunledun A, Alli AO, Webber MA. High Levels of Multidrug Resistance in Clinical isolates of Gram-negative Pathogens from Nigeria. International Journal of Antimicrobial Agents. 2011;37:62–66.

Husna A, Rahman MM, Badruzzaman ATM., Sikder MH, Islam MR, Rahman MT., Alam J, Ashour HM. Extended-Spectrum β -Lactamases (ESBL): Challenges and Opportunities. Biomedicines. 2023; 11:2937.

Available:https://doi.org/10.3390/biomedicines11112937

Joël TE, Bernardin AA, Karine GKM, Nathalie GK, Simon-Pierre NA. Detection and Dissemination of Extended-Spectrum Beta-Lactamases Genes (CTX-M-15 and SHV-187) Isolated in Multi-Drug Resistant Uropathogenic Klebsiella pneumoniae and Escherichia coli in Cote D’ivoire. Journal of Advances in Biology & Biotechnology, 2022;25(7);20–29. Available:https://doi.org/10.9734/jabb/2022/v25i7586

Bassey A.P, Ngwai Y.B, Nkene I.H, & Tama S.C. Molecular Characterization of plasmid-mediated extended spectrum beta-lactamase resistance in urinary Escherichia coli from patients in General Hospital, Maitama, Abuja, Nigeria. Asian Journal of Biotechnology and Genetic Engineering. 2024;7(1):43–53.

Available:https://journalajbge.com/index.php/AJBGE/article/view/123

Fu P, Luo X, He L, Rong H, Li C, Chen S, Zhang L, Wang A, Wang C. The molecular and epidemiological characteristics of carbapenemase-producing Enterobacteriaceae isolated from children in Shanghai, China, 2016–2021. Journal of Microbiology, Immunology and Infection. 2023;56(1):48-56.