Effects of Cnidoscolus aconitifolius on Some Hematological Parameters of Male Wistar Rats

Main Article Content

Ijeoma Ezebuiro
Chibuike Obiandu
Friday Saronee
Ikechukwu I. Weleh
Adesua C. Obiandu

Abstract

Introduction: Medicinal plants have become increasingly useful as a form of alternative therapy. Cnidoscolus aconitifolius is a medicinal plant applied in folklore remedies in the treatment and prevention of various diseases.

Aim: The aim of this study is to assess the effects of Cnidoscolus aconitifolius on some hematological parameters of male Wistar rats.

Methodology: A total of 15 male Wistar rats weighing between 100-250 g were randomly placed in groups. Group 1 served as control and received distilled water only; group 2 received 200 mg/kg and group 3 received 400 mg/kg of the hydromethanolic leaf extract of Cnidoscolus aconitifolius. Extract was administered once daily using oro-gastric cannula for 30 days. Blood samples were collected by direct cardiac puncture into appropriate sample tubes for estimation of hematological parameters including red blood cell count (RBC), packed cell volume (PCV), haemoglobin (Hb) concentration, white blood cell count (WBC) and platelet count.

Results: Results showed a significant (P<0.05) increase in RBC, PCV, Hb and platelet count with the higher dose of 400 mg/kg body weight of the extract compared to control group. However, the WBC count was not significantly (P>0.05) altered.

Conclusion: Oral administration of the leaf extract of Cnidoscolus aconitifolius increases red blood cell count, packed cell volume, hemoglobin level and platelet count at 400 mg/kg body weight.

Keywords:
Cnidoscolus aconitifolius, hematological parameters, hydromethanolic, wistar rats.

Article Details

How to Cite
Ezebuiro, I., Obiandu, C., Saronee, F., Weleh, I. I., & Obiandu, A. C. (2020). Effects of Cnidoscolus aconitifolius on Some Hematological Parameters of Male Wistar Rats. Asian Journal of Biotechnology and Bioresource Technology, 6(3), 1-5. https://doi.org/10.9734/ajb2t/2020/v6i330080
Section
Original Research Article

References

Iwu M. Handbook of African Medicinal Plants. CRC Press Boca Raton, FL. 1993; 32-38

Chilpa R, Baggio CH, Solano AD, Muniz E, Kauffman FC, Sanchez RI. Inhibition of gastric H+, K+-ATPase activity by flavonoids, coumarins and xanthones isolated from Mexican medicinal plants. Journal of Ethnopharmacology. 2006;105: 167-172.

Stern A. Drug-induced oxidative denaturation in red blood cells. Semin hematology. 1989;26:301-306.

Josef B. Phenylhydrazine haematotoxicity. Journal of Applied Biomedicine. 2007;5: 125-130

Awoyinka AO, Balogun IO, Ogunnowo AA. Phytochemical screening and in vitro bioactivity of Cnidoscolus aconitifolius (Euphorbiaceae). Journal of Medical Plant Research. 2007;1(3):063-065.

Institute for Laboratory and Animal Research. Guide for the care and use of laboratory animals. Seventh Edition. National Academies press. Washington DC, USA; 1996.

Akachukwu D, Okafor PN, Ibegbulem CO. Phytochemical content of cnidoscolus aconitifolius and toxic effect of its aqueous leaf extract in wistar rats. Journal of Investigational Biochemistry. 2014;1(1): 26-31.

Kurner D, Grimm E. The determination of blood parameters by Reflotron. Tierarztl Prax. 1989;17(1):101-103.

Bowman WC, Rand MT. Textbook of Pharmacology. Oxford: Blackwell Publishers. Drugs affecting coagulation, fibrinolysis, haematopoiesis and the functioning of blood cells. 1980;21(1–21): 53.

Erslev AJ, Gabuzda TG. Pathologic physiology, mechanisms of diseases. In: Sodeman WA, Sodeman TM, editors. 6th Ed. Philadelphia: WB Saunders Company. 1979;587–741.

Jakubowski JA, Thompson CB, Vaillancourt R, Valeri CR, Deykin D. Arachidonic acid metabolism by platelets of differing size. British Journal of Haematology. 1983;983:503–11.

Giles H, Smith RE, Martin JF. Platelet glycoprotein IIb/IIIa and size are increased in acute myocardial infarction. European Journal of Clinical Investigation. 1994;24: 69–72.

Ganong WF. 22nd ed. Singapore: McGraw Hill; Review of Medical Physiology. 2005; 515–7.

Almamary MA. Antioxidant activity of commonly consumed vegetables in Yemen. Malasian Journal of Nutrition. 2002;8:179-189.

Imoru JO, Eno AE, Unoh FB, Enkanu E, Ofem OE, Ibu JO. Hematopoetic agents in the crude extracts from the leaves of Viscum album (mistletoe). Nigerian Journal of Health and Biomedical Sciences. 2005; 4(2):139-145.

Bendich A. Physiological role of antioxidants in the immune system. Journal of Diary Science. 1993;76:2789-2794.