Degradation of Crude Oil by Bacteria Isolated from Various Soil Plantation at Idanre, Nigeria
Asian Journal of Biotechnology and Bioresource Technology,
Degradation of crude oil by bacteria isolated from three plantations soil at Idanre, Nigeria were comparatively investigated. Soil samples collected by hand trowel at 5cm rhizosphere of Cocoa, Orange, and Teak plantations from Idanre, Ondo State. The crude oil – forcados blend was obtained from Warri, Delta State, Nigeria. Gram negative bacteria were isolated from rhizosphere soil sample using standard microbiological methods. Preparation of Biomass was done by centrifuging nutrient broth repeatedly to wash cells. Harvested cells were obtained for the degradation of crude oil. Harvested cells were inoculated with crude oil and then incubated in a shaker. Degradation of crude oil was monitored by using spectrophotometer to read the OD at 540 nm. The bacteria isolated from rhizosphere of cocoa include: Citrobacter freundii, Yersinia pestis, Edwardsiella tarda,. Serratia marcescens. Rhizosphere of orange; Providencia stuatii while Enterobacter agglumerans, Moellerella wisconsis were isolated from rhizosphere of teak. The most effective hydrocarbon utilizing bacteria was Enterobacter agglumerans, which resulted in increase in population densities and reduction in hydrocarbon contents in the crude oil. Findings from this study shows the effectiveness of degrading hydrocarbon in liquid medium and further confirmed the potency of bacterial cells to degrade crude oil.
- Crude oil
How to Cite
James G, Speight. The Chemistry and Technology of Petroleum (Fourth ed.). CRC Press; 2006.
Gary JH, Handwerk GE. Petroleum Refining Technology and Economics (2nd ed.). Marcel Dekker, Inc;1984.
America's climate choices: panel on advancing the science of climate change; national research council. Advancing the Science of Climate Change. Washington, D.C.: The National Academies Press; 2010.
Rajasekar A. Biodegradation of petroleum hydrocarbon and its influence on corrosion with special reference to petroleum industry. In: Heimann K, karthikeyan O, Muthu S. (eds) biodegradation and bioconversion of hydrocarbons. Environmental Footprints and Eco-design of Products and Processes. Springer, Singapore; 2017.
Nilanjana Das, Preethy Chandran. Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnology Research International; 2011;13.
Article ID 941810
Atlas R, Bragg J. Bioremediation of marine oil spills: when and when not-the Exxon Valdez experience. Microbial Biotechnology.2009;2(2):213-221.
Grangemard IJ, Wallach R, Marget-Dana F, Peypous. Applied Biochemistry Biotechnology. 2001;90-199.
Lloyd CA, Cackette TA. Air and waste management association. 2001;51: 805.
Patrick-Iwuanyanwu KC, Onyemaenu CC, Wegwu MO, Ayalogu EO. Hepatotoxic and Nephrotoxic Effects of Kerosene and Petrol-Contaminated Diets in Wistar Albino Rats. Research Journal of Environmental Toxicology. 5(9):49-57.
Bhattacharya D, Sarma PM, Krishnan S, Mishra S, Lal B. Applied Environmental. Microbiology. 2002;5(69):1435.
Rathi V, Yadav V. Oil Degradation Taking Microbial Help and Bioremediation: A Review. J Bioremediat Biodegrad. 2019;10:460.
Van Hamme JD, Singh A, Ward OP. Recent advances in petroleum microbiology. Microbiol Mol Biol Rev. 2003;67(4):503-549.
Nwaogu LA, Onyeze GOC, Nwabueze RN. African Journal of Biotechnology. 2008;20:754-1939.
Fawole MO, Oso BA. Laboratory manual on microbiology. Spectrum Books Limited, Ibadan, Nigeria. 2007;127.
Kumar CG, Mamidyala SK. Extracellular synthesis of silver nanoparticles using culture supernatant of Pseudomonas aeruginosa Colloids and Surfaces B: Biointerfaces. 2012;84:462-466.
Latha R, Kalaivani R. Bacterial degradation of crude oil by gravimetric analysis. Advances in Applied Science Research. 2012;3(5):2789-2795.
Wolfe BE, Klironomos JN. Breaking new ground: Soil communities and exotic plant invasion. Bio Science. 2005;55(6):477-487.
Mittal Y, Chisti and Banerjee U. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances. 2013; 31:346-356.
Barathi S, Vasudevan N. Utilization of petroleum hydrocarbons by Pseudomonas fluorescence isolated from a petroleum-contaminated soil. Environ. Int. 2001;26:413-416.
Rastogi M, Nandal M, Khosla B. Microbes as vital additives for solid waste composting. Heliyon. 2020;6(2):e03343. Published 2020
Ollivier B, Magot M. Petroleum microbiology. 1st ed. ASM Press: Washington, DC. 2005;365.
Brooijmans W, Pastink M, Siezen J. Hydrocarbon-degrading bacteria: the oil- spill clean-up crew. Microbial Biotechnology. 2009; 2(6):587– 594.
Salleh AB, Ghazali FM, Zaliha RN, Basri MM. Biodegradation of crude oil polluted soil. Indian Journal of Biotechnology.2003;2:411-425.
Rosenberg E, Ron EZ. Bioremediation of petroleum contamination in Crawford RL and Crawford DL” (eds.) Cambridge: Cambridge University Press. Bioremediation: Principles and Application. 1996;100-124.
Salleh AB, Ghazali FM, Zaliha RN, Basri MM. Biodegradation of crude oil polluted soil. Indian Journal of Biotechnology. 2003;2:411-425.
Abstract View: 90 times
PDF Download: 39 times