Asian Journal of Biotechnology and Bioresource Technology https://journalajb2t.com/index.php/AJB2T <p><strong>Asian Journal of Biotechnology and Bioresource Technology (ISSN: 2457-0125)</strong> aims to publish high quality papers (<a href="https://journalajb2t.com/index.php/AJB2T/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of Biotechnology and Bioresource Technology. By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> <p><strong>NAAS Score: 4.66 (2024)</strong></p> SCIENCEDOMAIN international en-US Asian Journal of Biotechnology and Bioresource Technology 2457-0125 Effects of Commercial Feeding with Different Proteins on the Growth of G6 Transgenic Mutiara Catfish in Budikdamber Rearing https://journalajb2t.com/index.php/AJB2T/article/view/200 <p>This research was conducted in the Hatchery Building 4 Faculty of Fisheries and Marine Science, Padjadjaran University. The implementation started from July to September 2023. The purpose of this study was to test the growth performance of G6 transgenic mutiara catfish by feeding commercial feed containing different proteins to induce growth rate and protein use efficiency. The treatment design used the complete randomized design (CRD) method with four treatments (A: 39% feed protein; B: 32% feed protein; C: 20% feed protein; and D: 37% feed protein (mixture of pindang tongkol and PF.1000)) with three replications. Data were analysed using Sigmaplot 15.0 for absolute weight gain (Wg), feed conversion ratio (FCR), protein retention (RP), and protein productive value (PPV) and using Duncan Multiple Range Test (DMRT) with 95% confidence level. The results showed that treatment A gave the best results on the value of Wg (average weight gain of 4619 g ± 344.3 g) and the lowest FCR (0.63 ± 0.058), and the best protein retention and PPV values were shown in treatment B, as follows 19.24% and 17.4%.</p> Ibnu D. Buwono Roffi G.H Kiki H Ahmad A.R Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-03-23 2024-03-23 10 2 1 10 10.9734/ajb2t/2024/v10i2200 Identification of Antibiotics Susceptibility Patterns at Sub-inhibitory Concentrations in Pseudomonas aeruginosa https://journalajb2t.com/index.php/AJB2T/article/view/201 <p><em>Pseudomonas aeruginosa </em>is a Gram negative bacterium that has been recognized as an opportunistic pathogen. It is the most common bacterium associated with nosocomial infections and ventilator-associated pneumonia. It exhibits high innate resistance to various ranges of antibiotics thereby causing high morbidity and mortality rate. This research was aimed to identify the antibiotics susceptibility patterns at sub-inhibitory concentration in <em>Pseudomonas aeruginosa</em>. One hundred and fifty (150) clinical swab specimens were collected from urinary catheters; wound and ear infections of patients and the swabs inoculated using standard microbiology method. The isolates were characterized based on the bacteriological methods such as morphology and biochemical tests. The isolates were further confirmed by species specific by PCR amplification of 16S rRNA and the amplicons were analyzed by gel electrophoresis; and further genomic sequencing was done and blast with NCBI database mining. The antimicrobial susceptibility of the isolates was done by disc diffusion methods. The result of the isolation showed 22(59.46%) from wound infections, 12(32.43%) from ear infections and 3(8.11%) from urinary catheter. The isolates were Gram-negative, produced β-hemolysis on blood agar and the morphology is small pigmented circular. The isolates showed positive results to catalase, oxidase, citrate, nitrate and indole tests. The amplification of the 16S rRNA gene region resulted in the band size of 1500bp PCR product and the BLAST analysis gave 99% similarity. The results of susceptibility analysis showed that the isolates from the urinary catheter, wound and ear infection were 82%, 68% and 47% respectively, resistance to Piperacillin tazobactem, Cefoperazon, Ofloxacin, Tetracycline, Amikacin, Gentamycin, Bacitracin, Clarithromycin, Cefalotin, Levofloxacin and Cefpiroma. Antimicrobial susceptibility tests in <em>P. aeruginosa</em> isolates revealedshowed that they were multi-drug resistant. At sub-inhibitory concentrations of antibiotics within the microbial environment, <em>Pseudomonas aeruginosa</em> becomes more resistant. Perhaps, these antimicrobials could have other signaling activities within the environment. Therefore, there is a need for more research work to develop therapeutics combination to combat the recalcitrant nature of <em>Pseudomonas aeruginosa.</em></p> C. C. Williams G. A. Ajibade V. M. Y. Dan Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-03-26 2024-03-26 10 2 11 19 10.9734/ajb2t/2024/v10i2201 Phylogenetic Analysis of Bacterial Isolates Recovered from Salt-affected Soils of Haryana & Punjab, India https://journalajb2t.com/index.php/AJB2T/article/view/202 <p>Excessive saline/alkaline conditions present a significant challenge to the environment and ecology, impacting yield, plant growth, and soil health. This study focuses on isolating and characterizing halophilic bacteria from salt-affected areas in Haryana and Punjab, India. Morphological, biochemical, and molecular analyses were conducted to assess their potential as plant growth-promoting rhizobacteria (PGPR) for mitigating salt stress in salt-affected soils. Four bacterial strains, identified as HR3-PM, PB01-KB, PB-424, and PB-466, were isolated and characterized. Morphological and biochemical assays revealed diverse traits among the isolates, including phosphate solubilization, indole-3-acetic acid (IAA) production, and ammonia excretion. Molecular identification via 16S rRNA sequencing confirmed their taxonomic classification and revealed close homology to known bacterial species such as <em>Klebsiella aerogenes</em>, <em>Pseudomonas</em> <em>mosselii, </em><em>Lysinibacillus acetophenoni</em>, and <em>Pseudomonas stutzeri</em>. Phylogenetic analysis provided insights into their evolutionary relationships. These salt-tolerant bacteria exhibit promising PGPR activities, suggesting their potential for sustainable agriculture and soil remediation practices in salt-affected soils. Harnessing their abilities could offer cost-effective and environmentally friendly solutions to mitigate soil salinity, enhance plant productivity, and contribute to global environmental stresses. Further research is warranted to fully understand and harness the biotechnological potential of these halophilic bacteria in salt-affected ecosystems, paving the way for a more sustainable environmental future.</p> Parul Bhatt Kotiyal Shivam Kumar Sharma Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-03-28 2024-03-28 10 2 20 32 10.9734/ajb2t/2024/v10i2202 Micropropagation of Turmeric (Curcuma longa L.) on Large-Scale https://journalajb2t.com/index.php/AJB2T/article/view/203 <p><strong>Aim: </strong>Turmeric (<em>Curcuma longa</em> L.) is a slow-to-reproduce perennial herb that is propagated from ground rhizomes. Despite its well-known medicinal and culinary uses, its supply for propagation is limited due to disease susceptibility and slow rhizome development.</p> <p><strong>Methodology:</strong> To optimize turmeric bud cluster development and determined appropriate concentrations of BAP (6-benzyl aminopurin) and NAA (α-naphthalene acetic acid) for enhancing shoot multiplication and rooting rates.</p> <p><strong>Results: </strong>The results indicated that MS (Murashige and Skoog, 1962) was basic media for turmeric; shoot clusters formation on media supplemented with 1 mg L<sup>-1</sup>BAP and 0.5 mg L<sup>-1</sup> NAA; for shoot multiplication was 3 mg L<sup>-1 </sup>BAP and 0.5 mg L<sup>-1 </sup>NAA; and 0.5 mg L<sup>-1 </sup>NAA was favored for root induction</p> <p><strong>Conclusion: </strong>This research contributed to advancing our knowledge of valuable plants in Vietnam, particularly turmeric production on large-scale.</p> Cao Sang Nguyen Ngoc Hanh Dung Bui Thanh Hoa Pham Hong Diep Tran Van Minh Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-05-03 2024-05-03 10 2 33 44 10.9734/ajb2t/2024/v10i2203 Effects of Plant Growth Regulators on Micropropagation of Black Pepper (Piper nigrum) in Commercial-Scales https://journalajb2t.com/index.php/AJB2T/article/view/204 <p><strong>Aim: </strong>Black pepper (<em>Piper nigrum</em>) is renowned as a popular spice used to flavor dishes around the world and is also an agricultural export commodity that brings high economic value to many countries. However, traditional propagation methods by seed, cuttings, layering, and grafting reveal many limitations such as the low multiplication coefficient, the low survival rate of seedlings, and especially when there is an outbreak of disease, the ability to control the disease source is exceedingly difficult, leading to the spread of disease. Therefore, an alternative method through apical meristem micropropagation has been applied to contribute to sustainable and disease free pepper cultivars. Propagation by apical shoot meristem gives high propagation efficiency, good and uniform seedling quality, and can be propagated in large numbers in a brief period.</p> <p><strong>Methodology: </strong>This study is conducted to contribute to optimizing the process of micropropagation of pepper plants to meet the needs of producing excellent quality seedlings, serving the Vietnamese pepper industry. The two steps in the micropropagation sequence studied in this project are shoot formation and bud cluster multiplication. In this study, we investigate the effects of BA (6-Benzylaminopurine) and NAA (α-Naphthalene acetic acid) in shoot formation. Regenerated shoots were then cultured in MS media supplemented with different combinations of Kinetin (6-Furfurylaminopurine) and NAA in bud cluster multiplication experiment.</p> <p><strong>Results: </strong>The results showed that the apical shoot meristem cultured on MS medium (Murashige and Skoog, 1962) supplemented with 0.5 mg L-1 NAA and 2.0 mg L-1 BA was the optimal medium for shoot formation rate (100%). Besides, MS culture medium supplemented with Kinetin 1.5 mg L-1 + NAA 0.5 mg L-1 produced superior results in bud cluster multiplication compared with other treatments (8, 20 buds) after 8 weeks (about 2 months).</p> <p><strong>Conclusion:</strong> BA, Kinetin and NAA give important rules on micropropagation of black pepper in commercial-scale.</p> Nguyen Ba Dat Tran Thi Hoang Anh Bui Thanh Hoa Pham Hong Diep Tran Van Minh Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-05-18 2024-05-18 10 2 45 59 10.9734/ajb2t/2024/v10i2204 Effect of Culinary Treatments on Nutritional and Anti- nutritional Profils of Sesame Oilcake for Use in Fighting Protein Malnutrition https://journalajb2t.com/index.php/AJB2T/article/view/205 <p>To tackle protein malnutrition, the valorization of sesame cake has been initiated. A by-product of sesame oil extraction, it is commonly used as livestock feed. However, it could be used to enrich staple foods and improve their nutritional value, particularly in rural areas where access to quality protein sources is limited. In order to highlight its nutritional richness, certain soaking, roasting and hulling treatments were used to assess their impact on nutritional quality. Then extraction of the oil from the seeds using a mechanical press to obtain sesame cake was proceeded. Standard methods were used for physicochemical characterization of nutritional, mineral and anti-nutritional compounds. The results showed that the sesame oilcake obtained from the various treatments contained significant levels of total protein (26.55- 36.72g/100g DM), residual lipids (24.19- 32.37g/100g DM), carbohydrates (10.86- 18.74g/100g DM), ash (4.32 - 6.19 g/100g DM) and fiber (10.71-20.76 g/100g DM). Similarly, evaluation of the mineral composition of these meal concentrates showed their richness in phosphorus (20.47 - 176.66 mg/100g DM), calcium (15.75 - 467.42 mg/100g DM), magnesium (13.45 - 340.33 mg/100g DM), iron (4.90-14.70 mg/100g DM), and zinc (0.71-4.39 mg/100g DM). However, these sesame cake concentrates also contained anti-nutritional factors such as oxalates (0.48 - 1.04 mg/100g DM), phytates (0.08 - 0.12 mg/100g DM), saponins (0.084 - 1.10 mg/100g DM) and tannins (0.33 - 1.36 mg/100g DM). Fortunately, these were considerably reduced by pretreatment. Indeed, a 40.07, 83.33, 64.64 and 60.95% reduction in tannins, phytates, oxalates and saponins respectively were observed. The considerable reduction in anti-nutrients in the various cakes is an advantage for the digestibility and nutrient availability of this feed. Its high protein and mineral content could therefore be considered for use in protein malnutrition.</p> Bebbe Fadimatou Lonodjita Trésor Doumta Charles Falang Moundipa Fewou Paul Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-05-19 2024-05-19 10 2 60 71 10.9734/ajb2t/2024/v10i2205 Assessment of Airborne Extended Spectrum β-Lactamase Producing Enterobacteriaceae Isolates from Motor Parks in Benin City, Nigeria https://journalajb2t.com/index.php/AJB2T/article/view/206 <p><strong>Aim:</strong> The study was aimed at assessing airborne bacteria and extended spectrum β-lactamase producing Enterobacteriaceae isolated from the motor parks in Benin City.</p> <p><strong>Methodology:</strong> Airborne bacteria samples were collected in the motor parks (Central, Ugbowo, Agip, 19<sup>th</sup> Street, and Ekosodin Park), in Benin City using the settled plate method on Nutrient and MacConkey agar. Temperature, relative humidity, and airborne bacteria of the different parks were determined. The bacterial isolates were identified using phenotypic method. The antibiotic susceptibility profile was investigated by the disc diffusion method. The Enterobacteriaceae isolates were screened for Extended-spectrum β-lactamase (ESBL) production potentials. Virulence factor analysis and detection of ESBL genes (TEM, CTX-M, SHV, and OXA) were carried out on the selected ESBL producers.</p> <p><strong>Results: </strong>The relative humidity recorded at the parks at the point of agar plate exposure ranged from 82&nbsp; ̶&nbsp; 90%. The airborne heterotrophic bacterial and coliform counts from the motor parks ranged from 9.50±0.63 × 10<sup>2</sup>&nbsp; ̶&nbsp; 1.18±0.02 ×10<sup>3 </sup>cfu/m<sup>3</sup> and 2,48±0.41×10<sup>2</sup>&nbsp; ̶&nbsp; 3.30±0.41×10<sup>2 </sup>cfu/m<sup>3</sup> respectively. The identified Enterobacteriaceae were <em>Citrobacter freundii, Serratia marcescens, Citrobacter koseri, Klebsiella pneumoniae, Morganella morganii, Klebsiella oxytoca, Shigella dysenteriae, Proteus mirabilis, Escherichia coli, Enterobacter cloacae </em>and<em> Citrobacter diversus</em>. The isolate with highest frequency of occurrence was <em>E. coli</em> with values of 30.48 %. The isolate <em>K. pneumoniae</em> had the highest MAR index of 0.8. The isolate <em>S. marcescens</em> had the highest virulent factor of 60% and possessed all the ESBL genes detected.&nbsp;</p> <p><strong>Conclusion:</strong> Strong infection control measures should be put in place because more ESBL-producing Enterobacteriaceae have emerged in the surroundings of specific parks in Benin City.</p> Cyprian E. Oshoma Frank M. Akro Patrick O. Akpoghelie Osayi B. Isichei-Ukah Solomon E. Omonigho Copyright (c) 2024 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2024-06-11 2024-06-11 10 2 72 84 10.9734/ajb2t/2024/v10i2206