Micro-propagation and Appraisal of Hereditary Constancy through RAPD Analysis of Eclipta alba: A Significant Curative Plant Species
Priyanka Priyadarsini
College of Basic Science and Humanities, OUAT, Bhubaneswar- 751003, India.
Suchinnata Swapnasarita Sardar *
Department of Botany, Rama Devi Women’s University, Vidya Vihar, Bhubaneswar-751022, India.
Sudha Suman
Department of Botany, Rama Devi Women’s University, Vidya Vihar, Bhubaneswar-751022, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: To set up a prudent and standard protocol to frame in vitro recovery of Eclipta alba, a therapeutically significant plant, from nodal district by shoot culture initiation.
Study Design: Our examination was designed arbitrarily and treatment had threefold samples of 30 explants each. All components including number of shoots per explants, shoot length, root length and so forth were determined from the complete number of explants from which cultures were set up. The contaminated cultures were not regarded. Data were analyzed by using the “Statistical Analysis Software (SAS)” and all the information were directed to one-way classified analysis of variance (ANOVA) and means of treatments were compared based on Turkey’s honestly significant different test (HSD) at “0.01 probability level” using SAS.
Place and Duration of Study: Department of Botany, College of Basic Science and Humanities, and Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, between July 2019 and December 2020.
Methodology:
- Surface sterilization and preparation of explants
- Preparation of Culture medium and culture conditions for in vitro plant regeneration
- Acclimatization of regenerated plantlets
- Assessment of genetic fidelity through PCR based RAPD investigation
Results: BAP is the most effective Plant Growth Regulator for regeneration in E. alba and MS medium supplemented with BAP and Kin the foremost successful combination for multiple shoot proliferation. Soil: vermicompost (1:1) mixture has shown the best promising result of hardening. All the plantlets developed through in vitro culture have attended genetic stability with the mother plant. There is no genetic variation shown through RAPD analysis.
Conclusion: It is a successful attempt to generate a quick, economic and reproducible protocol for the micropropagation of E. alba without any genetic alternation and maintaining homologous clonal propagation.
Keywords: In vitro micro-propagation, Eclipta alba, genetic fidelity, RAPD, BAP, NAA