Plant Tissue Culture Regeneration and Aseptic Techniques
E. E. Ikenganyia *
Department of Agronomy and Ecological Management, Enugu State University of Science and Technology, Agbani, Enugu State, Nigeria.
M. A. N. Anikwe
Department of Agronomy and Ecological Management, Enugu State University of Science and Technology, Agbani, Enugu State, Nigeria.
T. E. Omeje
Department of Agricultural Technology, Enugu State Polytechnic, Iwollo, Enugu State, Nigeria.
J. O. Adinde
Department of Horticultural Technology, Enugu State Polytechnic, Iwollo, Enugu State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The science or art of growing plant cells, tissues or organs isolated from the mother plant on artificial media is known as plant tissue culture. The merits of plant tissue culture in crop production is that it helps in disease elimination, quick international distribution of genetic resources, germplasm conservation and reduction in quarantine requirements, time and space of regeneration. This paper looks at the materials for in vitro plant tissue culture, different methods of plant tissue culture media preparations, protocols of hygiene and sterilization in plant tissue culture and techniques in plant tissue culture for crop improvement.
Keywords: Tissue culture, in vitro, media, explants, cell