Retracted: Bioactive Compounds Identified from Cellulytic Bacteria Isolated from Food Dissipated and Their Screening for Potential Antimicrobial and Cytotoxicity Activities
M. R. Mahmud *
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Sajib Podder
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.
Rima .
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Food waste is a rich source of lingo-cellulosic materials thus providing an intense environment for the growth of cellulolytic bacteria. The present study was conducted to isolate and identify bioactive compound of cellulose degrading bacteria from food waste and their screening for potential antimicrobial activity. Cellulytic bacteria Klebsiellia pneumoniaee strain ZJ-02 identified was isolated from food waste. To test the sensitivity of the isolates, five different antibiotics were used. The strains were tested for resistance to Neomycin 30µg, Ampicillin 10mg, Cephalosporin 30µg, Doxycycline 30µg, and Erythromycin 15µg. When tested by disc diffusion method on antibiotic spreaded nutrient agar plate the strains showed sensitivity to Neomycin, Ampicillin, Cephalosporin, Doxycycline, and Erythromycin. The ethyl acetate extract of Klebsiellia pneumoniae strain ZJ-02 showed cytoxicity against brine shrimp nauplii with the LD50 value of 50.486 µg/ml and showed potent anti-bacterial activity against five human pathogenic bacteria Listeria monocytogenes, Bacillus subtilis, Shigella boydii, Escherichia coli, Klebsiella pneumoniae. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. The MIC and MBC values were between 32-64μg/ml and 64-128μg/ml. Higher MBC value than that of MIC indicates Klebsiellia pneumoniae strain ZJ-02 is bacteriostatic. In addition, potential active compounds were detected using gas chromatography and mass spectrometry- 1,2-benzenedicarboxylicacid, Pyrrolo[1,2-a]pyrazine-1,4-dione, 3,6-Diisobutyl 2,5piperazinedion, trimethylsilyl 3,5-dimethoxy-4-(trimethylsilyloxy) benzoate, 3H-cycloocta[c]pyran-3-one and phthalic acid were found in EAEKP. In summary, our findings provide insight into the Klebsiellia pneumoniae strain ZJ-02 from food waste are a potential source of novel antibiotic leads that are worthy of further study.
Retraction Notice: This paper has been retracted from the journal. This journal is determined to promote integrity in research publication. This retraction is in spirit of the same. After formal procedures editor(s) and publisher have retracted this paper on 25th November-2019. Related policy is available here: http://goo.gl/lI77Nn
Keywords: Cellulolytic bacteria, 16S rRNA, 1, 2-benzenedicarboxylicacid, Pyrrolo[1,2-a]pyrazine-1,4-dione, 3H-cycloocta[c]pyran-3-one, phthalic acid.